Abstract
Bacterial isolation from oil-contaminated and uncontaminated soil was screened for hydrocarbon utilizer which was also capable of producing lysine. Microbial production of lysine by Microbacterium lacticum was investigated in submerged fermentations using various concentrations of hydrocarbon, sugar sources and nitrogen. Of the nine sugar and five nitrogen sources tested, glucose/ammonium sulphate proved optimum for lysine production. Effect of varying concentration of carbon and nitrogen sources on lysine accumulation showed that glucose (4%) ammonium sulphate (1%) respectively increased lysine production. A gram positive rod bacterium identified as Microbacterium lacticum was identified. Optimizing the cultural conditions of Microbacterium lacticum in submerged medium gave a methionine yield of 2.99 mg/ml lysine in the broth culture after 96 h.
Highlights
Lysine is one of the nine essential and commercially important amino acids, out of the twenty naturally occurring amino acids
The present results show a marked improvement of the lysine fermentation by hydrocarbon utilizer, in its yield as well as the fermentation time
The fermentation process for the production of lysine by Microbacterium lacticum was successfully assessed in shake flasks on a laboratory scale
Summary
Lysine is one of the nine essential and commercially important amino acids, out of the twenty naturally occurring amino acids. It is a basic building block of all protein. Lysine cannot be synthesized biologically in the body and its breakdown is irreversible [1], but may be added to food and feed materials to improve the protein quality [2].
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