Lysine and methionine supplementation ameliorates high inclusion of soybean meal inducing intestinal oxidative injury and digestive and antioxidant capacity decrease of yellow catfish

Abstract

The yellow catfish Pelteobagrus fulvidraco with initial average weight 16.6±0.17g were fed three extruded diets for 56days. Fish meal (FM) diet was formulated as the normal control with 380g FM and 200g soybean meal (SBM) kg-1 diet. The SBM diet was prepared with 220g FM and 360g SBM kg-1 diet without Lys or Met supplementation. The SBM supplement (SBMS) diet was similar to SBM diet and supplemented with Lys and Met to ensure their levels similar to FM diet. The results showed fish fed SBM diet had lower percent weight gain and specific growth rate than the other two groups (P<0.05). Whole body protein content of fish fed FM and SBMS diets were higher than that of fish fed SBM diet (P<0.05). The hepatosomatic and intestosomatic indexes of fish fed SBM diet were significantly lower than that of fish fed FM and SBMS diets (P<0.05). The activities of pepsin in stomach, trypsin, and chymotrypsin in intestine, alkaline phosphatase and creatine kinase in proximal intestine, Na+, K+-ATPase, and gamma-glutamyl transpeptidase in distal intestine were significantly higher in fish fed FM and SBMS diets compared to SBM diet. The activities of catalase, glutathione-S-transferase, reduced glutathione, superoxide anion scavenging, and hydroxyl radical scavenging in the intestine showed the same changes (P<0.05). Malondialdehyde and protein carbonyl contents in intestine were significantly decreased in fish fed SBMS diet compared to SBM diet. These results indicated high inclusion of SBM induced intestinal oxidative injury and digestive and antioxidant capacity decrease. The Lys and Met supplementation could ameliorate these adverse effects on yellow catfish.