Abstract
In traditional Algerian medicine, Pinus halepensis (Mill.) resin is used as antimicrobial, anti-inflammatory, analgesic, wound healer, for the treatment of respiratory and urinary diseases. In the present study, a lyophilized aqueous extract (LAE) of Pinus halepensis (Mill.) resin is subjected to chemical composition analysis by gas chromatography coupled to mass spectrometry (GC/MS), antioxidant activity was evaluated in vitro using 2,2-diphenyl-b,picrylhydrazyl (DPPH) and β-carotene bleaching assays. LAE of Pinus halepensis (Mill.) resin was also tested for its potential activity against four dermatophytic fungi: Trichophyton rubrum, Trichophyton equinum, Trichophyton mentagrophytes, Trichophyton tonsurans. Results showed that LAE of Pinus halepensis (Mill.) resin contained 64.57% of aromatic compounds. This extract contains also succinic acid, sugars and alcohol sugars, alkaloids and other compounds that represent respectively 0.58%, 6.45%, 0.87% and 2.05%. The obtained extract exhibited satisfying antioxidant activity with an inhibition percentage of 93.76±0.41 at a concentration of 300 µg/mL and IC50 of 83.64µg/mL by the DPPH method. The tested extract showed also a good antioxidant activity using β-carotene test. At resin concentration of 100 µg/mL antifungal tests showed, inhibition rates of 58.9%, 58.44%, 41.36% and 34.43% against T. tonsurans, T. equinum, T. rubrum and T. mentagrophytes respectively. Upon the obtained results, this extract can be used as external treatments for wounds to prevent oxidation and fungal infections.
Highlights
Plants have been used as an important source of medicine since ancient times and their products are being used for different purposes such as medicine, food, health care, agriculture, agrochemicals, pharmaceutical, etc
A lyophilized aqueous extract (LAE) of Pinus halepensis (Mill.) resin is subjected to chemical composition analysis by gas chromatography coupled to mass spectrometry (GC/MS), antioxidant activity was evaluated in vitro using 2,2-diphenyl-b,picrylhydrazyl (DPPH) and β-carotene bleaching assays
Analysis of the chemical composition of the LAE of pine resin by GC/MS resulted in the identification of 53 compounds.The most representative results according to the NIST database (Table 1), are aromatic compounds such as cinnamic and benzoic acids derivatives that represent, quantitatively the most important fraction
Summary
Plants have been used as an important source of medicine since ancient times and their products are being used for different purposes such as medicine, food, health care, agriculture, agrochemicals, pharmaceutical, etc. Natural coniferous resins extracts are raw materials for various products in the industry and have been used as traditional medicines as a homemade salve for skin wounds and infections (Sipponen, 2013). Resin infusion and decoction for the treatment of respiratory ailments (bronchitis, pneumonia and colds), urinary problems, parasitosis and wounds (Miara et al, 2019; Bendif et al, 2020). Several studies reported that the most important fungal species implied in dermatophytic diseases in Algeria belonged to Tricophyton genus. They were predominant in the Northeast, 66.68% including Trichophyton rubrum and Trichophyton tonsurans (Hammadi et al, 2007). In the last decade most research done on pine in Algeria were focused on the organic solvent extraction of essential oils from aerial parts of the tree, spines (Abi-Ayad et al, 2011), needles (Fekih et al, 2014; Dob et al, 2005), twigs and buds (Fekih et al, 2014), flowering aerial parts (Haichour et al, 2020) or pine
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