Abstract
Background: Platelet-rich fibrin (PRF) has gained popularity in craniofacial surgery, as it provides an excellent reservoir of autologous growth factors (GFs) that are essential for bone regeneration. However, the low elastic modulus, short-term clinical application, poor storage potential and limitations in emergency therapy use restrict its more widespread clinical application. This study fabricates lyophilised PRF (Ly-PRF), evaluates its physical and biological properties, and explores its application for craniofacial tissue engineering purposes. Material and methods: A lyophilisation method was applied, and the outcome was evaluated and compared with traditionally prepared PRF. We investigated how lyophilisation affected PRF’s physical characteristics and biological properties by determining: (1) the physical and morphological architecture of Ly-PRF using SEM, and (2) the kinetic release of PDGF-AB using ELISA. Results: Ly-PRF exhibited a dense and homogeneous interconnected 3D fibrin network. Moreover, clusters of morphologically consistent cells of platelets and leukocytes were apparent within Ly-PRF, along with evidence of PDGF-AB release in accordance with previously reports. Conclusions: The protocol established in this study for Ly-PRF preparation demonstrated versatility, and provides a biomaterial with growth factor release for potential use as a craniofacial bioscaffold.
Highlights
Platelet-rich fibrin (PRF) has gained popularity in craniofacial surgery and dentistry due to the absence of anticoagulant and xeno-origin components. It provides a rich reservoir of autologous growth factors (GFs) that are essential for bone regeneration, including (1)
The present study demonstrates the application of lyophilisation as a novel method The present study demonstrates the application of lyophilisation as a novel method for PRF preservation, primarily for maintaining the physical microarchitecture of PRF and for PRF preservation, primarily for maintaining the physical microarchitecture of PRF and preserving autologous platelet-derived growth factors (PDGF)-AB growth factor release
lyophilised PRF (Ly-PRF) may be favourable for bone regeneration, where Ly-PRF can be used as an autologous growth factor reservoir or as a non-weight-bearing autologous bioscaffold within craniofacial tissue
Summary
Platelet-rich fibrin (PRF) has gained popularity in craniofacial surgery and dentistry due to the absence of anticoagulant and xeno-origin components. The search for new approaches to preserve the biological function of platelets is considerably important; lyophilisation or a freeze-drying method was proposed to address the current drawbacks of the short clinical half-life of fresh platelet concentrates [10]. In this context, PRF lyophilisation, i.e., freezing followed by water sublimation and the subsequent removal of water vapour, was proposed as a consistent method for fabrication for an off-the-shelf product with improved stability, ready for future application [10]. SEM, and (3) the release kinetics of PDGF-AB using ELISA
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
