Abstract
In previous communications 1 we have described the isolation of the virus of lymphogranuloma inguinale from the pus and glandular tissue of a patient with the inguinal type of the disease and its successive transmission through white mice. We have also found, confirming Wassen's 2 observations, that lymphogranulomatous mouse brains offer an excellent source of highly potent and specific antigen for the performance of the Frei test. This work has been repeated recently by Lichtenstein and von Haam. 3 The purpose of the present paper is to record the results of one year's clinical use of antigen prepared from mouse brain for the diagnosis of lymphogranuloma inguinale. Prior to the introduction of material from experimental animals the only antigen available was that prepared from pus from a human being, as first described by Frei. 4 This original antigen consisted of an emulsion in a physiologic solution of sodium
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