Abstract
We have studied bone marrow lymphocytes subpopulations in 67 patients with breast cancer and 13 patients with benign processes in the mammary gland. Morphological study of the cellular composition of the bone marrow and immunological analysis of lymphocyte subpopulations have been done. Comparative characterization of lymphocyte subpopulations in the bone marrow of patients with breast cancer and benign breast diseases was done by flow cytometry. The gate of Mature lymphocytes (CD45++) studied subpopulations of T cells (CD3), b cells (CD19), NK cells (CD56+CD3–). Among T lymphocytes (CD3+) was studied TCRγδ and TCRαβ cells, a subpopulation of CD4, CD8, CD4+, CD25+ T-regulatory cells. Within В-lymphocytes (CD19+) was studied subpopulations CD10, CD38, CD5 lymphocytes. We revealed a significant increase of CD5+, CD38+ B-cells in patients with breast cancer compared to benign tumors. Index of maturation of erythrocytes was significantly higher in BC 0.96 in comparison with benign processes 0.92. When comparing immunological data with histological structure of the tumor in the group of breast cancer patients, it was noted that the percent of CD10+ B-lymphocytes was significantly higher in infiltrative ductal cancer in comparison to infiltrative lobular carcinoma (19.3 and 8.0 % accordingly; p = 0.024).
Highlights
We have studied bone marrow lymphocytes subpopulations in 67 patients with breast cancer and 13 patients with benign processes in the mammary gland
Comparative characterization of lymphocyte subpopulations in the bone marrow of patients with breast cancer and benign breast diseases was done by flow cytometry
We revealed a significant increase of CD5+, CD38+ B-cells in patients with breast cancer compared to benign tumors
Summary
Блохина»; Россия, 115478, Москва, Каширское шоссе, 23. Исследованы субпопуляции лимфоцитов костного мозга 67 больных раком молочной железы (РМЖ) и 13 пациенток с доброкачественными процессами в молочной железе. Выполнена сравнительная характеристика субпопуляций лимфоцитов костного мозга больных РМЖ и пациентов с доброкачественными заболеваниями молочных желез методом проточной цитометрии. Индекс созревания эритроцитов был достоверно более высоким при РМЖ (0,96) в сравнении с доброкачественными процессами (0,92). У больных РМЖ среднее содержание плазмоцитов костного мозга было более низким в сравнении с пациентками, имеющими доброкачественные образования. При сопоставлении иммунологических данных с гистологическим строением опухоли в группе больных РМЖ отмечено, что содержание СD10+ В-лимфоцитов было достоверно более высоким при инфильтративно-протоковом раке в сравнении с инфильтративно-дольковым (19,3 и 8,0 % соответственно; р = 0,024). Ключевые слова: рак молочной железы, стернальная пункция, субпопуляции лимфоцитов костного мозга, проточная цитометрия, иммунологический анализ, лимфоциты
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
