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Abstract
SUMMARY One-way mixed leukocyte culture (MLC) tests were performed using, in all cases, mitomycin C-treated stimulating cells from healthy university students. Responding cells from four groups were tested: hospitalized patients with malignancy, age- and sex-matched hospitalized patients without known malignancy, age- and sex-matched nonhospitalized controls, and students who were younger than the persons in the other three groups. Whereas approximately 30% of the variation in response was unaccounted for by known variables, the great majority of the remaining variation could be accounted for by the number of polymorphonuclear leukocytes present in the culture. These results suggest that quantitation of MLC tests may be better after removal of polymorphonuclear leukocytes from the culture—a step we have now instituted in our laboratory.
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