Abstract
Introduction: Diabetes mellitus has remained one of the serious health problems in the world; and oxidative stress has been reported to be a root cause for the progression and development of diabetes mellitus and its associated complications. Aim: This study investigated the possible ameliorative effects of lycopene on diabetic-induced changes in erythrocyte osmotic fragility and lipid peroxidation in Wistar rats. Methodology: The animals were made diabetic by single intraperitoneal injection of streptozotocin at 60 mg/kg b w. Diabetes was confirmed by the presence of high fasting blood glucose level ≥ 200 after 72 hours. Thereafter, diabetic rats were randomly assigned into six groups (1, 2, 3, 4, 5 and 6) comprising five animals each. Group 1 (Diabetic control) and group 2 (Normal control) rats received 0.5 ml of olive oil, groups 3, 4, 5 rats received 10, 20, 40 mg/kg bw of lycopene respectively, while those in group 6 received 2 mg/kg bw of glibenclamide orally once daily for a period of four weeks. At the end of the treatment, all animals were sacrificed; blood samples collected for determination of erythrocyte osmotic fragility (EOF) and lipid peroxidation (LPO). Results: The results obtained showed that there was a significantly (P < 0.05) lowered erythrocyte osmotic fragility in diabetic animals treated with lycopene when compared with diabetic control group. In addition, there was also a significantly (P < 0.05) reduced erythrocyte malondialdehyde concentration, an index of lipid peroxidation in the diabetic treated groups when compared with diabetic control group. Conclusion: From the available findings, it can be concluded that administration of lycopene to diabetic rats attenuated diabetic-induced changes in EOF and LPO and these observed effects may be attributed to anti-oxidative property of lycopene.
Highlights
Diabetes mellitus has remained one of the serious health problems in the world; and oxidative stress has been reported to be a root cause for the progression and development of diabetes mellitus and its associated complications
Aim: This study investigated the possible ameliorative effects of lycopene on diabetic-induced changes in erythrocyte osmotic fragility and lipid peroxidation in Wistar rats
From the available findings, it can be concluded that administration of lycopene to diabetic rats attenuated diabetic-induced changes in erythrocyte osmotic fragility (EOF) and lipid peroxidation (LPO) and these observed effects may be attributed to anti-oxidative property of lycopene
Summary
Diabetes mellitus has remained one of the serious health problems in the world; and oxidative stress has been reported to be a root cause for the progression and development of diabetes mellitus and its associated complications. Aim: This study investigated the possible ameliorative effects of lycopene on diabetic-induced changes in erythrocyte osmotic fragility and lipid peroxidation in Wistar rats. At the end of the treatment, all animals were sacrificed; blood samples collected for determination of erythrocyte osmotic fragility (EOF) and lipid peroxidation (LPO). Results: The results obtained showed that there was a significantly (P < 0.05) lowered erythrocyte osmotic fragility in diabetic animals treated with lycopene when compared with diabetic control group. Hyperglycaemia is mediated in large part, by a state of enhanced oxidative stress, which results in the excessive production of reactive oxygen species These reactive oxygen species cause both adverse structural and functional changes in tissues [6] including red blood cells. Oxidative stress, mediated mainly by hyperglycemia-induced generation of free radicals, contributes to the development and progression of diabetes mellitus and its related complications. Previous studies in a cell-free system have suggested that in hyperglycaemia, glucose can enolize and thereby reduce molecular oxygen yielding a-keto aldehydes and free radical intermediates [7]
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