Abstract
Abstract Promyelocytic zinc finger transcription factor (PLZF) is an important transcription factor expressed by natural killer T (NKT) cells and a variety of other innate T cells. Development of these PLZF positive cells require the adaptor molecule SLAM- associated protein (SAP) and the interaction of the signaling lymphocyte activation molecule (SLAM) family member receptors in addition to TCR stimulation. However, it remains unclear how these interactions influence the development of innate thymocytes. We have found that costimulation of pre-selection double positive (PS-DP) thymocytes with an agonist to the SLAM family receptor Ly108 greatly enhances PLZF expression compared to TCR stimulation alone. Furthermore, PLZF expressing cells were reduced in Ly108 deficient mice and increased in transgenic mice expressing a hyperactive Ly108 isoform. Costimulaiton with Ly108 also increased the expression of Egr-2 and the binding of Egr-2 to the PLZF (Zbtb16) promoter. Surprisingly costimulation with CD28 was unable to enhance Egr-2 nor PLZF expression in vitro suggesting that Ly108 has a unique role in PLZF expression. This work provides a novel mechanism by which innate thymocyte development is regulated via Ly108 and possibly other SLAM family receptor interactions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
