LW‐AFC Attenuates LPS‐Induced Neuroinflammation via Inhibiting Nuclear Factor‐ Kappa B Signaling

Abstract

Neuroinflammation and activation of microglia have been recognized as important pathological events in Alzheimer's disease (AD). LW‐active fraction combination (LW‐AFC) is extracted from Liuwei Dihuang decoction (LW), a classic traditional Chinese medicinal prescription. It has been demonstrated that LW‐AFC had neuroprotective effects on AD animal models. However, whether anti‐inflammatory effect contributes to the neuroprotection of LW‐AFC has not been fully elucidated. The present study aimed to evaluate the effects of LW‐AFC on neuroinflammation and its underlying mechanisms. C57 mice were intraperitoneally injected with lipopolysaccharide (LPS) and treated with LW‐AFC (i.g.). The results revealed that LW‐AFC significantly improved the hippocampal long‐term potentiation (LTP) and decreased the contents of tumor necrosis factor‐α (TNF‐α) and interleukin‐1β (IL‐1β) in the serum and hippocampus of the LPS‐treated mice. In vitro study showed that LW‐AFC inhibited LPS‐induced activation of BV‐2 cells and markedly reduced the increases of TNF‐α and IL‐1β levels in the supernatant of LPS‐treated BV‐2 cells. In addition, LW‐AFC downregulated the expression level of NF‐κB in LPS‐treated BV‐2 cells. The present findings indicate that LW‐AFC might attenuate neuroinflammation both in animal and cell model, which may contribute to its neuroprotective effect in AD treatment.Support or Funding InformationProject supported by the National Natural Science Foundation of China (81503078)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.