Luxury zinc supply acts as antiaging agent and enhances reproductive fitness in Arabidopsis thaliana

Abstract

Developmental senescence in plants is an age dependent process affected by phytohormones, nutrient status, and environmental factors, while the antiaging effects of zinc are recognized in humans. This study explores the possible influence of a high, non-toxic Zn-supply (12 μM) on senescence and reproductive fitness in A. thaliana. Auxin-resistance mutant, axr1–12, and auxin overexpressing YUCCA6 mutant, yuc6−1D, and their corresponding background genotypes were grown until complete rosette senescence to quantify the fruit biomass and seed number. Gene expression of different antioxidant, auxin and senescence-associated markers were analyzed after the onset of senescence. All mutants showed delayed developmental senescence. Luxury Zn delayed senescence in wild type, but not in the mutant genotypes. Excluding axr1−12 mutants, which showed very low expression of the auxin gene marker INDOLE-3-ACETIC ACID INDUCIBLE 2 (IAA2), enhanced expression of the senescence markers SENESCENCE-ASSOCIATED GENE 12 (SAG12) and AUXIN RESPONSE FACTOR 2 (ARF2) coincided with decreased expression of IAA2. Delayed senescence and total number of seeds per plant were related to higher expression of the peroxisomal antioxidant enzymes Cu/Zn superoxide dismutase (SOD3) and catalase (CAT2). These results evidence that high Zn-induced delayed senescence and improved reproductive fitness in Arabidopsis are related to an auxin-independent mechanism that retains antioxidant activity.