LUTEOLIN REDUCES CARDIAC DYSFUNCTIONS AND MITOCHONDRIAL OXIDATIVE STRESS IN STREPTOZOTOCIN‐INDUCED DIABETIC RATS

Abstract

AimTo investigate the effects of luteolin on cardiac functions and mitochondrial oxidative stress in streptozotocin (STZ)‐induced diabetic rats.MethodsMale Sprague‐Dawley rats were randomly divided into a normal control group, a luteolin control group, a diabetic group, and diabetic groups orally administered with a low dose (10 mg/kg/d) or a high dose of luteolin (100 mg/kg/d) for eight weeks. The body weight, blood glucose, cardiac functions, left ventricular weight, myocardial collagen, and reactive oxygen species (ROS) levels were assayed. The cardiac mitochondrial ROS level, superoxide dismutase (SOD) activity and the mitochondrial swelling were measured.ResultsTreatment with luteolin had no effect on the blood glucose but reduced the losing of body weight in diabetic rats. High dose of luteolin markedly reduced the ratio of ventricular weight and body weight, increased the left ventricular develop pressure, and decreased the left ventricular end diastolic pressure in diabetic rats. The myocardial levels of ROS and collagen, the cardiac mitochondrial ROS level, and the mitochondrial swelling in diabetic rats were all markedly reduced by high dose of luteolin. Furthermore, high dose of luteolin significantly increased the mitochondrial SOD activity in diabetic rat hearts.ConclusionTreatment with luteolin for 8 weeks markedly improves the cardiac function, which may be related to reducing mitochondrial oxidative stress and mitochondrial swelling, in diabetic rats.This work was supported by grants from the National Natural Science Foundation of Zhejiang Province ( Y206179 ).