Abstract

1. 1. Neuraminidase, a bacterial enzyme, was used to selectively destroy follicle-stimulating hormone (FSH) activity of chicken anterior pituitary glands. 2. 2. The enzyme-treated pituitary material and various other pituitary hormones were tested to determine their effect on 32P uptake by chick testes. Adrenal cortical stimulating hormone (ACTH), growth hormone (GH) and prolactin did not increase 32P uptake. 3. 3. Treatment with 1 mg of the enzyme-treated pituitary material resulted in a 150 per cent increase in 32P uptake. This response was attributed solely to LH. 4. 4. An assay based on bovine LH standards (NIH-LH-B5) demonstrated that the LH activity of chicken anterior pituitary glands could be measured within the limits of 1·0 to 8·0 gmg.

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