Luteinizing Hormone Receptor Gene Polymorphism of Pasundan Cattle in Ciamis

Abstract

Pasundan cattle is a local livestock that has lived and well adapted in West Java. In 2015, the population declined by 20.96% caused by the high selling prices and changes in land functions. The impact of declining of the population can causes genetic degradation indirectly. One of the efforts to increase the population is selection of cattle based on luteinizing hormone receptor gene polymorphism that can used as molecular selection for reproductive traits. Thirty seven heads of Pasundan cattle with artificial insemination calving records was used in this study. The exon 11 of LHR gene was amplified and subsequently analysed by sequencing method. The sequencing result were found three genotypes of CC, CT and TT in SNP g.1337C>T. The frequency of T allele (0.527) shows more dominant over C allele (0.473). The chi square test showed that Pasundan cattle were in Hardy-Weinberg equilibrium. The heterozygosity value of Ho (0.513) indicating that the heterozygosity value of the LHR gene belongs to high category (>50%). The high genetic diversity of the Pasundan cattle population indicates that there has not been selection program yet. The results of this study can be used as a reference to continue the selection program improving genetic quality in the reproductive traits of Pasundan cattle.