Lung protective effects of stellate ganglion block on mechanical ventilation in rabbits

Abstract

Objective To evaluate the effects of stellate ganglion block(SGB) on rabbit ventilator associated lung injury (VALI). Methods Forty rabbits were randomly divided into two groups(n=20): the control group (groupⅠ) and the SGB group(group Ⅱ). Group Ⅰ is only given NS. Group Ⅱ is stellate ganglion block. Wet/dry(W/D), activities of superoxide dismutase(SOD), myleoperoxidase(MPO), and level of malondialdehyde(MDA) in lung tissue were detected at the time of 1 h(T1), 2 h(T2), 4 h(T3), 6 h(T4) after mechanical ventilation. MDA, TNF-α and SOD in serum were measured. Results W/D and activities of SOD in groupⅡ were both significantly lower than groupⅠ [(6.07±0.12) vs (8.58±0.48), (64±10) U/mg vs (77±11) U/mg](P<0.01), level of MDA and activities of MPO were significantly higher than groupⅠ [(0.89±0.12) μmol/g vs (0.63±0.11) μmol/g, (0.46±0.09) U/g vs (0.28±0.07) U/g](P<0.01). The lung tissue lesion showed from HE staining under light microscope and electron microscope in groupⅡ was lighter than group I. Compared with groupⅠ , serum MDA and TNF-α of the rabbits in groupⅡat T2-T4 time points were significantly lower (P<0.05). Compared with groupⅠ , serum SOD in groupⅡ at T2-T4 time points significantly rised(P<0.05). Conclusions SGB reduced the injury of lung tissue in rabbits with mechanical ventilation and had a protective effect to lung. Key words: Sympathetic nerve block; Ventilator associated lung injury; Rabbit