Lung Cancer Cellular Apoptosis Induced by Recombinant Human Endostatin Gold Nanoshell‐Mediated Near‐Infrared Thermal Therapy

Abstract

Recombinant human endostatin (rhES)can inhibit tumor cell proliferation and apoptosis. The effect of proliferation and apoptosis of A549 lung cancer cells induced by gold nanoshell particles of recombinant human endostatin (G‐rhES) with near‐infrared (NIR) irradiation was studied. Gold nanoshell particles were prepared. Endostatin was connected with the bond A‐U through surface modification by bioconjugation of core‐shell structured gold nanoshells. Proliferation of A549 lung cancer cells induced by G‐rhES was detected by MTT. Cellular apoptosis was researched by flow cytometry. Mitochondrial membrane potential was investigated using a confocal microscope. Morphological changes were studied by atomic force microscopy. Under irradiation in the 820 nm NIR, the proliferation of A549 lung cancer cells was significantly induced by G‐rhES. NIR gold nanoshell particles showed synergism with endostatin, which may be related to hyperthermia‐increased apoptotic effect of endostatin. Our study results suggest that G‐rhES can inhibit lung cancer cells proliferation.The new treatment strategy of G‐rhES combined with thermal therapy may lead to lung cancer remission. Concentration (ug/ml) 24h 48h 72h Inhibition rate (%) Inhibition rate (%) Inhibition rate (%) 0 ‐ ‐ ‐ 2.5 1.2 2.4 3.4 5 4.0 5.9 6.3 10 4.4 6.3 10.2 20 13.7 15.8 17.8 40 16.8 18.5 25.1 80 20.7 25.6 30.1 Concentration (ug/ml) Apoptotic rate (%) 0 0.9+—0.1 10 5.4+—2.3 20 15.9+—4.9 40 21.4+—2.8 imageimage