Luminescent ZnO quantum dots for sensitive and selective detection of dopamine

Abstract

Water-soluble and luminescent ZnO quantum dots (QDs) capped by (3-aminopropyl) triethoxysilane (APTES) are environment-friendly with strong photoluminescence (max. wavelength: 530nm). Interestingly, it was found that the fluorescence could be quenched by dopamine (DA) directly. On the basis of above, a novel ZnO QDs based fluorescent probe has been successfully designed to detect DA with high selectivity and sensitivity. Moreover, the possible fluorescence quenching mechanism was proposed, which showed that the quenching effect may be caused by the electron transfer from ZnO QDs to oxidized dopamine–quinone. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of DA within the range from 0.05 to 10μM, with the detection limit down to 12nM (n=3). Also, the selectivity experiment indicated the probe had a high selectivity for DA over a number of possible interfering species. Finally, this method was successfully used to detect DA in serum samples with quantitative recoveries (99–110%). With excellent selectivity and high sensitivity, it is believed that the ZnO QDs based fluorescent probe has a potential for the practical application in clinical analysis.