Abstract
Micropuncture samples from rat testis and epididymis were subjected to protein analysis, microelectrophoresis and ABP determinations. Rete testis fluid contained only 5% of the total protein concentration of blood serum. Other reproductive tract fluids contained one-third to one-half the total protein concentrations of blood. Electrophoresis of intratubular fluids from the various testicular and epididymal zones allowed separation of reproductive tract-specific protein bands in each fluid studied. Luminal fluids from the epididymal tubule contained several proteins not appearing in blood. Analysis for androgen binding activity revealed higher concentrations in rete testis fluid than in seminiferous tubule fluid. Caput epididymal fluid contained the highest ABP concentration.
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