Luffa rootstock enhances salt tolerance and improves yield and quality of grafted cucumber plants by reducing sodium transport to the shoot

Abstract

Soil salinity severely limits crop yield and quality. Grafting onto tolerant rootstocks is known as an effective means to alleviate salt stress. The present study was planned to find out the potential roles, mechanisms and applications of luffa rootstock to improve salt tolerance of grafted cucumber plants. Here, we screened a highly salt-tolerant luffa rootstock by evaluating the growth, photosynthetic performance, antioxidant defense and the accumulation of Na+ and K+ under salt stress. Reciprocal grafting between cucumber and luffa showed that luffa rootstock significantly improved the salt tolerance of cucumber plants, as evidenced by higher fresh weight, photochemical efficiency (Fv/Fm), and lower relative electrical conductivity (REC), which was closely associated with the decreased accumulation of Na+ and increased the accumulation of K+ in shoots of luffa grafted cucumber seedlings, leading to a lower Na+:K+ ratio in shoot when compared with self-grafted cucumber. Furthermore, grafting with intermediate stock of luffa also sufficiently alleviated cucumber salt stress by reducing Na+ accumulation in shoot and the whole plant but increasing Na+ accumulation in interstock and root under salt stress, fully proving the salt tolerance depending on the capacity of luffa interstock to limit the transport of Na+ from the root to the shoot. More importantly, luffa rootstock improved the growth, yield and quality of grafted cucumber plants grown in pots in solar greenhouse as revealed by increased net photosynthetic rate, plant height, leaf number, yield, Vitamin C and soluble sugar but decreased titratable acid under both salinity and normal conditions. Together, these results, for the first time, clearly demonstrated that luffa,a new highly salt-tolerant rootstock, enhances salt tolerance and improves yield and quality of grafted cucumber plants by reducing sodium transport to the shoot.