Abstract
A luciferase reporter assay is a cell-based, enzymatic experiment that utilizes an ectopically expressed luciferase in cultured cells or in live animals, with the presence of its substrate luciferin, to generate luminescence in response to gene regulation at the level of transcription. This assay can be easily formatted for high-throughput sample analysis. The reagents are commercially available and routinely used in various applications. We have automated a luciferase reporter assay on the Opentrons OT-2 platform to measure activation of NF-kB in HeLa cells. The Python script-based protocols were developed to perform cell seeding, reporter construct transfection, and enzyme-catalytic reaction to produce detectable signals for quantification with desirable quality of sample handling and minimal hands-on time.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
