Abstract
Objectives: This study aimed to taxonomically classify “Lubeg”, Philippine Cherry and molecularly identify this indigenous species thru DNA barcoding. Methods: Fresh samples of “Lubeg” leaves, unripe fruits and flowers were collected and submitted to Bureau of Plant Industry, Manila for authentication. The Key to Species of Syzygium Gaertn was used in the taxonomic classification of “Lubeg”. Molecular identification of the species used the plastid DNA (ptDNA) with two genes rbcL and matK, together with ITS 2. Leaf extracts were submitted to Macrogen, South Korea for the complete barcoding of the plant. The procedures were: gDNA extraction, PCR amplification, purification, sequencing and BLAST BI report. Results: “Lubeg”, Philippine Cherry belongs to family Myrtacaea under species Syzygium (Gaertn). It was identified as Syzygium lineatum (Roxb.) (DC.) Merr & Perry.‘Syzygium’ was derived from the Greek term ‘syzygos,’ meaning the paired leaves are opposite in arrangement and ‘lineatum’ means stripes, netted veins on the leaves. The amplification was only successful using the rbcL gene and furthermore, based on the sequences, the plant sample belongs to the genus Syzygium. It was noted that this was the first sequence of Syzygium lineatum under the rbcL gene. Using the phylogenetic tree, the “Lubeg” sequence is closest to Syzygium malaccense, and the out-group is Eucalyptus globulus. Improvements: The process of DNA barcoding with the same DNA markers be carried out with the same plant species to further verify the result of this study Keywords: DNA Barcoding, “Lubeg”, Molecular Identification, Taxonomic Classification
Highlights
The species of Syzygium lineatum is a native to Borneo, Cambodia, China (Guangxi), Philippines, Java, Laos, Malaysia, Myanmar, Small Suda Islands, Singapore, Sumatra, Thailand and Vietnam where it lives in humid forest[1]
Fresh samples of “Lubeg” leaves, unripe fruits and flowers were collected in Lal-lo, Cagayan
The Consortium for the Barcode of Life (CBOL) Plant Working group has forwarded the use of the plastid DNA genes rbcl and matK standard DNA barcode markers primarily because of the availability of published primers of these genes together with the high level of taxonomic resolution when these primers are used
Summary
The species of Syzygium lineatum is a native to Borneo, Cambodia, China (Guangxi), Philippines, Java, Laos, Malaysia, Myanmar, Small Suda Islands, Singapore, Sumatra, Thailand and Vietnam where it lives in humid forest[1]. In the Plant List, there are 1,374 scientific plant names of species rank for the genus Syzygium. Of these 1,123 are accepted names and one of these is Syzygium lineatum. “Lubeg”, “Malubeg” and “Alebadu” are the common names of Philippine Cherry, Syzygium lineatum(Roxb.). It inhabits some areas of Cagayan, Apayao and Isabela provinces of Region 02. In Cagayan, it is noted that “Lubeg” trees thrive mostly and abundantly in the municipality of Lal-lo
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
