Abstract
Easy and rapid quantification of hemoglobin (Hb) in the human body is essential for early screening of hematological disorders like anemia. Herein, we synthesized atomically precise tyrosine-protected photoluminescent silver nanocluster (Tyr-Ag NC) in a facile one-pot method. The synthesized nanocluster showed strong photoluminescence (PL) intensity peaked at 410 nm that can be selectively turned off by Hb with utmost sensitivity (limit of detection = 30 pM), thereby making the nanocluster a promising nano-probe for the detection of Hb. The observed PL quenching can be attributed to a complexation followed by a static charge transfer transition between the nanocluster and hemin, the cofactor of Hb involving both Fe2+ and the porphyrin ligand. The Fe2+ plays a key role in the complex formation which was disrupted by the formation of hydroxyl radicals through the Fe2+-H2O2 mediated Fenton reaction, restoring the PL intensity of the Tyr-Ag NC, ensuring its reusability for the detection of Hb. Compared to the conventional clinical techniques involving toxic chemicals and/or expensive equipment, the synthesized Tyr-Ag NC allows quick, direct, easy, accurate and cost-effective detection and quantification of Hb in real human serum and blood samples suggesting its tremendous practical applications for healthcare assessment.
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