<title>Spectroscopic research on purple sulphur bacteria Chromatium sp. in vivo</title>

Abstract

Phototrophic purple sulphur bacteria represent an important constituent of coastal zone biota and a crucial link of sulphur cycling in the nature. Purple bacteria are widespread in the environment occurring almost in every water basin and also in soil. The spectroscopic research was performed in vivo on purple sulphur bacteria Chromatium sp. in different culture development stages and illumination conditions during culture growth. Possibilities of purple bacteria quantification in vivo using absorbance and fluorescence intensities are described. The experiments revealed the possibility of application of the intensities ratio of porphyrin pigments emission to cell blue fluorescence for the estimation of the culture physiological status. These findings may be used for improvement of remote sensing techniques of ecological monitoring.