&lt;title&gt;Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner&lt;/title&gt;

Katsumasa Fujita,Tomoyuki Kaneko,Satoshi Kawata,Tetsuro Takamatsu,Osamu Nakamura,Masahito Oyamada

doi:10.1117/12.384225

<title>Real-time Ca ion wave imaging in living rat cardiac muscle cells by a confocal multiphoton microscope with a microlens-pinhole array scanner</title>

Katsumasa Fujita, Tomoyuki Kaneko + Show 4 more

https://doi.org/10.1117/12.384225

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Publication Date: Apr 27, 2000

Citations: 2

Affiliation: Osaka University, Kyoto Prefectural University of Medicine

#Rat Cardiac Cells #Cardiac Cells + Show 8 more

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Abstract

A real-time confocal multiphoton fluorescence microscope was developed to observe Ca2+ dynamics in living rat- cardiac muscle cells. The real-time imaging was achieved by multifocus excitation of a specimen with a rotating microlens-array disk. A pinhole-array disk for confocal detection was introduced in the microscope to improve the spatial resolution and the contrast of fluorescence images. Ca2+ wave and Ca2+ transient in cultured rat- cardiac cells were successfully observed with the developed microscope.

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