Abstract
Chemical sensing with fluorescent probes is usually accomplished by measuring the fluorescence intensity or using wavelength-ratiometric probes. Measurement of fluorescence decay times, rather than intensities, is advantageous because the decay times are largely independent of intensity changes due to light losses, photobleaching, or probe washout. Present technology allows measurement of nanosecond decay times by the phase-modulation method with inexpensive and robust instrumentation. This report describes the use of phase- modulation fluorometry for lifetime-based sensing of O2, pH, Ca2+ and K+.© (1992) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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