Abstract
A new method to detect and quantify ischemic states of tissue is presented. One important indicator of the metabolic and ischemic status of a cell is the concentration ratio of NADH : NAD<SUP>+</SUP>. The use of time-resolved laser- induced fluorescence techniques (LIF) in combination with previous biochemical measurements of NADH (Nicotineamide Adenine Dinucleotide) concentrations in tissue offer the possibility of quantitative NADH detection. The essential of the new method is a double-rescaling procedure of the fluorescence signal taking into account both the optical limitations of the device and the individually and locally varying optical tissue properties which are the scattering coefficient (mu) <SUB>s</SUB>, the absorption coefficient (mu) <SUB>a</SUB> and the anisotropy factor g. All the data presented in this paper were acquired from experiments with in vivo guinea pig hearts.
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