Abstract
Thiamethoxam is widely applied to control pest insects, but it causes serious environmental contamination. In this study, the degradation processes of the compound in soil and water collected from a rice field by native microorganisms, by mixed culture of Phanerochaete sp. Th1 and Ensifer sp. Th2, and by both the mixed pure culture and native microorganisms were conducted. The result showed that the mixed culture inoculation increased thiamethoxam in all media. In liquid media, the degradation percentage of the substrate in collected water was 21.8±4.4%, collected water + inoculation was 44.2±5.0%, and mineral medium + inoculation was 98.0±0.4%. Degradation determination in media with 70% collected water and 30% dry soil showed that the main degradation occurred in the liquid phase. The degradation rates in the media with 50% collected water and 50% dry soil were higher in the surface layer, and lower in the bottom. Indigenous microorganisms also played an important role in the degradation process. This study provided valuable information on the thiamethoxam degradation in simulated media of a rice field and the role of Phanerochaete sp. Th1 and Ensifer sp. Th2 to enhance the degradation.
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