Abstract
Williopsis beijerinckii IFO 0941 produced several acetic esters (ethyl, isobutyl, isoamyl and β-phenylethyl acetate) in a YPD medium. The production of acetic esters from alcohols and amino acids by W. beijerinckii IFO 0941 during fermentation was studied. It was found that isobutyl acetate was produced from L-valine and isobutyl alcohol, isoamyl acetate was produced from L-leucine and isoamyl alcohol, and β-phenylethyl acetate was produced from L-phenylalanine and β-phenylethyl alcohol, respectively. The production of acetic esters by W. beijerinckii IFO 0941 during fermentation was not increased by and addition of acetic acid. Moreover, contrary to the report of YOSHIZAWA that the production of acetic esters by Saccharomyces cerevisiae during fermentation was greatly reduced by unsaturated fatty acids added of the medium, the production of acetic esters by W. beijerinckii IFO 0941 was not inhibited by them.
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