Abstract

Electron-microscopic observations were made on M. glutamicus. 1. Polar granules which appear at some stages of incubation are thought to be volutin or metachromatic granules.2. Very small volutin granules were also observed in the cells which were cultured in glucose bouillon. Size of these granules were 100mμ-150mμ in diameter.3. Size of volutin granules increased to 300mμ-400mμ in diameter after 1.5h 7h culture in the synthetic medium.4. The thickness of the cell wall is less than 10mμ and its density is lower than that of cytoplasm.5. There are a few large vacuoles in cytoplasm.6. Low density part which was slightly fibrous was observed in cytoplasm, and may be considered to be the nuclear site.7. Usually, there are one to two vacuoles which contain a volutin granule of high electron density.8. Volutin granule is consisted of many small vesicles whose diameters are 5 mμ-20mμ.

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