Abstract
Purpose: To evaluate the effect of lysophosphatidic acid (LPA) on the proliferation, invasion and migration ability of 3AO, SKOV3 and CAOV3 human ovarian cancer cell lines. Methods: SKOV3, 3AO and CAOV3 cell lines were respectively treated with LPA. Changes in the proliferation rate of these cell lines were observed after LPA treatment. The cell lines that were not treated with LPA served as control group. Boyden chamber was used to assess cell invasion and migration capability. The expression levels of relevant cytokines related to cell migration in the supernatant of CAOV3 cell line were determined using ELISA following LPA stimulation. Results: The cell proliferation rate of human ovarian cancer cell lines was significantly accelerated after in vitro LPA treatment in a concentration-dependent fashion. Boyden chamber assay data indicate that invasion indices in 3AO and CAOV3 cell lines were significantly higher than those in untreated control cell lines (p < 0.05). However, no statistical significance was noted between 3AO and CAOV3 cell lines (p < 0.05). The expression levels of relevant cytokines in the CAOV3 cell line were significantly upregulated after LPA treatment (p < 0.05). Conclusion: LPA intervention in vitro accelerates cell proliferation rate and also significantly upregulates the expression levels of multiple cytokines related to cell migration in human ovarian cancer cell lines, suggesting that LPA plays a significant role in the invasion and migration of SKOV3, 3AO and CAOV3 cell lines. Keywords: Ovarian carcinoma, Tumor infiltration, Lysophosphatidic acid, Cell migration, Cytokines
Highlights
Lysophosphatidic acid (LPA) is a bioactive lipid mediator involved in tissue repair and wound healing
Levels of LPA are significantly increased in the plasma of ovarian carcinoma patients, indicating that LPA can promote the incidence of early events in ovarian carcinoma dissemination
LPA receptors belong to the endothelial differentiation gene (Edg) family, which consists of 4 categories
Summary
Lysophosphatidic acid (LPA) is a bioactive lipid mediator involved in tissue repair and wound healing. Human ovarian cancer cell lines including 3AO, CAOV3 and SKOV3 were selected and treated with different concentrations of LPA. The ovarian cancer cell lines 3AO, SKOV3 and CAOV3 were cultured in RPMI1640 complete culture solution containing 10 % fetal bovine serum at 37 °C and 5 % CO2. The ovarian cancer cell lines 3AO and CAOV3 were cultured in RPMI1640 complete culture solution containing 10 % fetal bovine serum. The CAOV3 cell line was cultured in serum-free solution for 12 h and treated with different concentrations of LPA (5, 20 and 40 μmol/L) for 16 h, and LPA was replaced by PBS in the control group. The treated cells in logarithmic growth phase were digested using 0.25 % pancreatin containing 10 % fetal bovine serum, transferred into the centrifuge tube and added by 100 μL culture solution.
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