&lt;i&gt;In vitro&lt;/i&gt; Antiglycation and Cross-Link Breaking Activities of Sri Lankan Low-Grown Orthodox Orange Pekoe Grade Black Tea (&lt;i&gt;Camellia sinensis&lt;/i&gt; L)

Wd Ratnasooriya,Tbs Muthunayake,Wksm Abeysekera,Cdt Ratnasooriya

doi:10.4314/tjpr.v13i4.12

Abstract

Purpose: To investigate the antiglycation and cross-link breaking activities of Sri Lankan low-grown orthodox Orange Pekoe grade black tea (Camellia sinensis L) Methods: Five concentrations (6.25, 12.5, 25.0, 50.0 or 100.0 µg/ml) of Black tea brew (BTB) were made using Sri Lankan low-grown Orange Pekoe (O.P.) grade tea. Antiglycation and advanced glycation end products (AGEs) cross-link breaking activities of BTB as well as the antiglycation activity of rutin were determined in vitro on bovine serum albumin/glucose system using fluorescence spectroscopy. Results: BTB induced significant (p<0.05) antiglycation activity (IC50, 19.04 ± 5.18 µg/ml) and AGEs cross-link breaking activities (IC50, 82.89 ± 3.44 µg/ml). These effects were dose-dependent. Further, the antiglycation activity of BTB was comparable to rutin, a well-known antiglycation agent (IC50, 21.88 ± 2.82 µg/ml). Conclusion: These results show that Sri Lankan low-grown O.P. grade black tea possesses both antiglycation and AGEs cross-link breaking activities in vitro and thus provides scientific justification for the use of black tea in Sri Lankan indigenous medicine for the management of diabetic complications.

Highlights

It is well known that diabetic complications are the major causes of morbidity and mortality among patients with type 2 diabetic mellitus
Since advanced glycation end products (AGEs) are involved in the pathogenesis of complications of diabetes and other disorders [15], several synthetic and natural products have been tested as inhibitors of AGEs formation and AGEs breakers as potential therapeutics for management of diabetic complications
Sieve analysis revealed that 83.5% of the tea particles were of true size (2000 – 4000 μm) for O.P. grade black tea

Summary

INTRODUCTION

It is well known that diabetic complications are the major causes of morbidity and mortality among patients with type 2 diabetic mellitus. Several natural products have been tested as potential inhibitors of AGEs formation [5,6] In this regard, if Sri Lankan black tea can attenuate diabetic complications as is claimed in indigenous medicine [7], it should possess potent antiglycation (inhibiting activity against AGEs formation) and/or cross-link breaking properties. If Sri Lankan black tea can attenuate diabetic complications as is claimed in indigenous medicine [7], it should possess potent antiglycation (inhibiting activity against AGEs formation) and/or cross-link breaking properties This has not been tested previously; antiglycation activity of black tea is largely unknown [5,6] as compared with those of green tea [5,6,8]. The freeze-dried product was stored in air-tight container at 4oC until use

Evaluation of antiglycation activity

RESULTS

DISCUSSION

CONCLUSION