Abstract
Effects of inorganic salts, sugars and amino acids were studied on the production and stability of killer toxin produced by a halotolerant killer yeast.Activity of the killer toxin in filtered culture borth of Debaryomyces hansenii TK-5 was increased by the addition of potassium chloride, magnesium chloride, potassium phosphate and calcium chloride, respectively, to the culture medium.Especially, the activity of the filtrate was increased 8 times by the addition of 200 mm potassium chloride to the culture medium. The heat and pH stability of the killer toxin was increased by the addition of sugar, sugar alcohol, ammonium sulfate and sodium chloride, respectively. The killer toxin was found to be markedly stabilized in the presence of 20 to 50% inositol. However, the activity of the killer toxin was inhibited by the addition of arginine, histidine and glutamic acid, respectively.
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