Abstract
Background: The use of non-allergic, non-toxic and eco-friendly natural dyes has become a matter of significant importance due to the increased environmental awareness in order to avoid some hazardous synthetic dyes. Method: The ethanolic extract of Curcuma longa was diluted using 1% acetic acid in 70% ethanol to a concentration of 0.2 g/ml. It was used to stain histological sections of the testes for 15 minutes. Curcuma longa was also used as a counter stain for Haematoxylin. Phytochemical constituents were investigated. Results: The Curcuma longa dye distinctly stained the seminiferous epithelium and interstitium yellow. Curcuma longa provided a good counter stain for Haematoxylin, taking up the acidic staining characteristics with Haematoxylin staining the basic staining characteristics. Phytochemical screening revealed the presence of saponins, alkaloids, tannins and flavonoids. Conclusion: Curcuma longa has good potential for use as a counter stain for Haematoxylin in the staining of tissues in lieu of Eosin.
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