Abstract
Cheirolophus intybaceus (Asteraceae, Centaureinae) or the constancy of 2C value.- Cheirolophus intybaceus is a heliophyte growing in thermal Mediterranean scrublands along a coastal belt of 50 km large, stretching from Toulon (France) to the Southern part of the Iberian Peninsula, occurring also in the Balearic Islands (with the exception of Minorca). Moreover, this species is also growing in high and sunny lands in the Mediterranean river basins, consti- tuting a complex of taxa closely related among them. The objectives of this work are: i) to provide new genome size data for some Asteraceae species; ii) to study the variation of DNA amount along a species distribution area; iii) to evaluate the discrimination capability of this parameter at low taxonomic levels. A signio cantly positive correlation between the DNA amount and the latitude has been found, that is, in drier and warmer habitats genome size tends to decrease in this species. The variation in the whole distribution area of Ch. intybaceus is 1.15-fold. This low variability supports the constancy of 2C-value. Abstract Cheirolophus intybaceus (Asteraceae, Centaureinae) or the constancy of 2C value.- Cheirolophus intybaceus is a heliophyte growing in thermal Mediterranean scrublands along a coastal belt of 50 km large, stretching from Toulon (France) to the Southern part of the Iberian Peninsula, occurring also in the Balearic Islands (with the exception of Minorca). Moreover, this species is also growing in high and sunny lands in the Mediterranean river basins, consti- tuting a complex of taxa closely related among them. The objectives of this work are: i) to provide new genome size data for some Asteraceae species; ii) to study the variation of DNA amount along a species distribution area; iii) to evaluate the discrimination capability of this parameter at low taxonomic levels. A signio cantly positive correlation between the DNA amount and the latitude has been found, that is, in drier and warmer habitats genome size tends to decrease in this species. The variation in the whole distribution area of Ch. intybaceus is 1.15-fold. This low variability supports the constancy of 2C-value.
Highlights
The variation in the whole distribution area of Ch. intybaceus is 1.15-fold
Se ha encontrado una correlación positiva y signi¿cativa entre la cantidad de ADN y la latitud, es decir, que en hábitats con menor pluviosidad y más cálidos el tamaño del genoma tiende a disminuir en esta especie
Molecular phylogeny of Cheirolophus (Asteraceae: Cardueae-Centaureinae) based on ITS sequences of nuclear ribosomal DNA
Summary
Juntament amb el seu origen, els recol·lectors i els números dels plecs d’herbari es troben a la Taula 1 i la distribució de les 45 poblacions estudiades es pot veure a la Fig. 1. Recol·lectors, data, testimoni d’herbari, contingut de DNA nuclear i latitud de les poblacions estudiades de Cheirolophus. Recol·lectors, data i testimoni d’herbari Espanya: País Valencià, Castelló, barranc d’Aigua Oliva, 13.12.2006, Aparicio, Garnatje 224 & Pellicer (BC). Espanya: País Valencià, València, Cullera, a les roques, 01.11.2006, Garnatje 204a & Pellicer (BC). Espanya: País Valencià, València, Cullera, al costat de la conducció d’aigua, 01.11.2006, Garnatje 204b & Pellicer (BC). Espanya: País Valencià, València, Pedralba, 01.11.2006, Garnatje 203 & Pellicer (BC). Espanya: País Valencià, Alacant, Benitatxell, cala Moraig, 03.11.2006, Garnatje 209 & Pellicer (BC). Espanya: País Valencià, Alacant, Benitatxell, urbanització "Cumbre del Sol", 03.11.2006, Garnatje 208 & Pellicer (BC). Espanya: País Valencià, Alacant, Xàbia, punta del cap Prim, 20.05.2005, Garnatje & Vilatersana 442 (BC). La quantitat total de DNA nuclear es calculà multiplicant la quantitat de DNA del patró intern pel quocient entre les dades numèriques de les posicions dels pics 2C de la mostra problema i del patró, assumint que hi ha una correlació lineal entre els senyals de Àuorescència dels nuclis tenyits de la mostra problema i del patró i el contingut de DNA (Fig. 2)
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