Abstract
The culture experiments of a maine centric diatom, Chaetoceros calcitrans f. pumilus TAKANO, were undertaken with compressed flasks of 100ml in volume under the following conditions:Temperature: 18.0-22.0°C, specific gravity of experimental culture medium containing the optimum value (52.6mgN/l) of KNO3: 23.44-25.40(σ15), light intensity of white fluorescent tube (National Hi-light) for culture: 2.5Klx (the radiant energy from the range of 400 to 700nm in light wave length was 4.3W and that from the whole light length wave was 23.3W), and bubbling volume: 1.5l/l/min.The results obtained were summarized as follows:1.The effective value of Na2HPO4 added to medium for increasing of ce11 numbers or packed cell volume (P.C.V.) of this diatom was 4.17-10.8mg P/l and its optimum value was 8.68mgP/l.2. The optimum value of Na2HPO4 added to medium for the increasing of P.C.V. was 8.68mgP/l. The increasing rate of P.C.V. in medium added 8.68mgP/l of Na2HPO4 was lower than that of cell numbers observed in the same medium.3. In the medium added more 13.0mgP/l of Na2HPO4, the flochs with many diatom cells occurred and the growth was inhibited.4. The N-P ratio of 12.6 in mg-atoms ratio or 6.06 in mg ratio seems to be the optimum N-P ratio for the excellent growth (increasings of cell numbers and P.C.V.)of this diatom.
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