Abstract
Among the genera that exhibit stem nodulation, Aeschynomene and Discolobium are found in the Pantanal Mato-grossense (wetlands in the state of Mato Grosso, Brazil). Isolates obtained from the stem and root nodules of D. pulchellum, D. psoraleaefolium, D. leptophyllum and A. fluminensis were collected from various locations in the Pantanal de Pocone sub-region and phenotypically characterized and genotyped by restriction analysis of the 16S rDNA gene. Of the 282 isolates obtained from the stem and root nodules, 84.3% alkalized YMA media and 74.1% showed slow growth. No differences in either the phenotype or the genotype among the rhizobial populations isolated from the root or stem nodules of the species Discolobium and A. fluminensis were observed. Among the isolates obtained from D. pulchellum, there was a group that was not similar to any of the reference strains used, and most of the isolates analyzed by PCR-RFLP were similar to the genus Bradyrhizobium.
Highlights
Prokaryotes that fix atmospheric nitrogen (N2) are an extremely important group of microorganisms for various ecosystems because they are responsible for the entrance of nitrogen into the ecosystem
The species D. pulchellum, D. psoraleaefolium and D. leptophyllum were found at the sampling points along the Transpantaneira Highway
All three species exhibited stem nodules, while root nodules were only obtained from D. psoraleaefolium and D. leptophyllum
Summary
Prokaryotes that fix atmospheric nitrogen (N2) are an extremely important group of microorganisms for various ecosystems because they are responsible for the entrance of nitrogen into the ecosystem. The ability to fix atmospheric nitrogen is widely distributed among prokaryotes with a range of phylogenetic relationships. The ability to fix atmospheric nitrogen and induce nodulation in legumes is restricted to members of the Proteobacteria phylum (MOULIN et al, 2001; SHIRAISHI et al, 2010). Et al, 2010), the Amazon Forest (JESUS et al, 2005; LIMA et al, 2009) and Cerrado (savanna) regions The Pantanal is the most extensive wetland region in the world. It is located in the Upper Paraguay River Basin of central South America and is composed of low-lying pluvial flatlands impacted by the rivers that drain into the basin. Due to the declivity of these lowlands, source water from the Paraguay River takes four months to flow through the Pantanal (ADÁMOLI, 1987)
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