&lt;b&gt;Effects of glutathione supplementation and carbon source during somatic embryogenesis of &lt;i&gt;Acca sellowiana&lt;/i&gt; (O.Berg) Burret (Myrtaceae)

Ana Flávia Pavei,Miguel Pedro Guerra,Leila Do Nascimento Vieira,Hugo Pacheco De Freitas Fraga

doi:10.4025/actascibiolsci.v40i1.40257

Abstract

The present study intended to investigate the effects of different glutathione (GSH) levels (0, 0.1, 0.5 and 1 mM) on the somatic embryogenesis (SE) induction of Acca sellowiana . Besides, we evaluated the effect of different carbon sources (sucrose and maltose) on the somatic embryos conversion. GSH-supplemented treatments resulted in improved SE induction rates (~70%) as compared to the control GSH-free (~35%) after 50 days of culture. The total number of somatic embryos obtained did not differ between treatments, but significant differences were observed for the embryonic stages after 80 days of culture. After 80 days of culture, 0.5 and 1 mM GSH-supplemented treatments showed the largest amount of torpedo-staged somatic embryos. In contrast, treatments supplemented with 0 and 0.1 mM GSH showed equal amounts of somatic embryos at all embryonic stages. These results indicate that GSH accelerates the SE induction process and increases the synchrony of the somatic embryo formation of A. sellowiana . The use of maltose for the somatic embryos conversion, as compared to sucrose, did not influence the conversion rate of normal chlorophyllous somatic embryos, but increased the formation of normal achlorophyllous somatic plantlets. This finding can be attributed to the rapid hydrolysis of sucrose, contributing to an enhanced chlorophyll synthesis.

Highlights

IntroductionThe development of its production would allow the processing of the fruits in products with high commercial value, such as juices, fermented and distilled beverages, ice cream and candied fruits (Vuotto et al, 2000; Ruberto & Tringali, 2004; Beyhan, Elmastaş, & Gedikli, 2010; Weston, 2010)
The total number of somatic embryos did not differ between the treatments evaluated after 80 days in culture, significant differences were observed in relation to the embryonic stages obtained in the different treatments (Table 2 and Figure 1)
Results found in the present study indicated that GSH supplementation improves the synchrony in the somatic embryo formation of A. sellowiana, increasing the number of somatic embryos in more advanced developmental stages (Table 2 and Figure 2)

Summary

Introduction

The development of its production would allow the processing of the fruits in products with high commercial value, such as juices, fermented and distilled beverages, ice cream and candied fruits (Vuotto et al, 2000; Ruberto & Tringali, 2004; Beyhan, Elmastaş, & Gedikli, 2010; Weston, 2010). This species impacts directly on the recovery of degraded land, riparian forests, permanent preservation areas, legal reserves, and constitution of agro-forest systems (Gomes, Oliveira, Ferreira, & Batista, 2016)

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Conclusion