Abstract
Grape berries metabolites determine wine grape and wine quality. The aim of this work was to determine the metabolite profiles of fruit of mature berries of ‘Cabernet Sauvignon’ of grapes in relation to their growing conditions in high alititude Shangeri-La wine-growing region. Last decade, Proton nuclear magnetic resonance (1H NMR) used to quantify in a single analysis many different compounds in berry extracts. Grape berries were harvested in 2013 on grapevines cultivated in Jinsha River and Lancan River of Shangeri-La, China. After an ethanolic-water extraction, the1H NMR spectra of water-soluble extracts of fruit berriers were run in 15 minutes. Bayesil was development a web system that automatically identifies and quantifies. Principal component analysis (PCA) analysis of berries spectra significantly discriminated mature berries from Jinsha River and Lancan River. 1H NMR spectra of berriers were less discriminanting. OPLS-DA showed significant grapevines cultivated in Jinsha River and Lancan River of Shangeri-La, contributing to the discrimination, were attributed to animo acid, organic acid, alcohol, sugar, Ester. In conclusion, 1H NMR analysis of berry extracts discriminates berries from different origins more efficiently than classical biochemical analyses based on sugar, acidity and Ester. alerting pathway of animo acid, organic acid pathway.
Highlights
Grapevines are economically important for wine production, and most of unique characteristics chemical compounds t are formed during fruit development [1]
The Jinsha River valley compared to the Lancan River valley showed an enhanced succinic acid pathway, which can function by adapting to CO2 partial pressure, improving the CO2 fixation rate, and resisting Citrobacter amalonaticus disease [16]
Cabernet Sauvignon grapes produced in the Jinsha River and Lancan River valleys were analyzed by 1D 1H Nuclear magnetic resonance (NMR) for metabolite profiling to clear the wine-growing terroirs in Shangeri-La
Summary
Grapevines are economically important for wine production, and most of unique characteristics chemical compounds t are formed during fruit development [1]. “terroir” has become an important characteristic to understand how the environment and human behaviors interact in wine development [3]. “Terroir” is a quality characteristic of grape wines in worldily wine-producing regions such as France, Italy, Spain, and Germany, and is defined as the interaction between the plants, the natural environment, and human behavior in vitiscuturepractice [4]. HPLC-MS is an attractive method with great potential for rapid and efficient screening of biomarkers [7]. Proton NMRspectroscopy is a powerful and useful tool for metabolite (1H NMR). 1H NMR is a nondestructive, noninvasive, and rapid method to determine grape Qualitative and quantitative analysis. 1H NMR is a nondestructive, noninvasive, and rapid method to determine grape
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