Abstract
Sixty healthy ovaries were collected from local slaughter house. Oocytes from small and medium sized follicles (2-8 mm in diameter) were selected for oocyte collection using aspiration followed by slicing . A and B category ( cumulus oocyte complexes (COCs) with more than 5 layers and 3-5 layers respectively of compact cumulus cells surrounding the zona pellucida) were selected for <italic>in vitro</italic> maturation. A total of 361 oocytes were found to be matured with overall mean maturation rate of 82.59 ± 0.02%. The mean number of grade A, B and C oocytes recovered per ovary was 4.26 ± 0.53, 3.05 ± 0.31 and 1.25 ± 0.19, respectively. The overall recovery of grade A, B and C oocytes were 256, 183 and 74 . Out of 439 oocytes 58.86 ± 0.05%, 33.23 ±0.04 % and 9.90 ± 0.04 % showed +++, ++ and + degrees of cumulus expansion, respectively. It was concluded that aspiration followed by slicing is an ideal method for maximum yield of oocytes and <italic>in vitro</italic> maturation in indigenous cow of Assam.
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