Abstract
The method is based on ternary complex formation among o -carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL−1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 104 dm3 mol−1 cm−1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.
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