<i>o</i>-カルボキシフェニルフルオロン-銅（II）錯体との三元錯体生成に基づくインスリンの吸光光度定量

Abstract

The method is based on ternary complex formation among o -carboxyphenylfluorone, copper(II), and insuline. In the determination of insuline, Beer's law is obeyed in the range of 0.5∼50 μg mL−1. The effective molar absorptivity at 550 nm and the relative standard deviation were 8.2 × 104 dm3 mol−1 cm−1 and 2.2% (n = 6), respectively. The proposed method should be useful for a simple determination of insuline.