Abstract
Purpose: To investigate the cognition-enhancing effects of aqueous extract of Indigofera tinctoria Linn (ITE, Fabaceae) in experimental amnesic mice. Methods : Scopolamine (2 mg/kg, i.p.) was used to induce amnesia in mice. The cognitive-enhancing activity of the ITE (5, 10 and 20 μg/mL) was studied by passive avoidance response, elevated plus maze and Y-maze behavioral paradigm in normal and scopolamine-induced amnesic mice. Antioxidant activities were also determined based on the ability of ITE to inhibit lipid peroxide, superoxide and hydroxyl radicals. Results : Scopolamine-induced cognitive deficits were significantly reversed by ITE (p < 0.001 at 20 mg/kg) in a dose-dependent fashion in all the behavioral paradigms tested. Furthermore, ITE dosedependently scavenged lipid peroxide, superoxide and hydroxyl free radicals with 50 % inhibition concentration (IC 50 ) of 7.28 ± 0.37, 5.25 ± 0.28 and 7.62 ± 0.43 μg/mL, respectively. Conclusion : ITE possesses cognitive-enhancing properties in amnesic mice due to its potent antioxidant action. Keywords : Indigofera tinctoria, Scopolamine, Lipid peroxidation, Amnesia, Antioxidant, Cognition
Highlights
Cognition in a broad sense means the ability of the brain to encode, store and retrieve information [1]
We evaluated cognitive-enhancing effects of I. tinctoria extract in amnesia-induced experimental mice models
There was no significant difference in transfer latency time (TLT) in the first trial among various groups
Summary
Cognition in a broad sense means the ability of the brain to encode, store and retrieve information [1]. We evaluated cognitive-enhancing effects of I. tinctoria extract in amnesia-induced experimental mice models. On the 15th day, after 60 min of administration of doses, acquisition trail for passive avoidance and the transfer latency for elevated plus maze was recorded. For Y-maze, animals received scopolamine 30 min before performing the test. Each animal was placed at the end of an open arm, facing away from the central platform. The reaction mixture (0.5 mL) containing mice brain homogenate (0.1 mL, 25 % w/v) in Tris-HCl buffer (40 mM, pH 7.0), KCl (30 mM), ferrous ion (0.16 mM), and ascorbic acid (0.06 mM) was incubated for 1 h at 37 °C in the presence and absence of various dilutions of ITE (1, 5, 10, 15 and 20 μg).
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