<i>In vitro</i> regeneration of grape

Abstract

Considering the global and Russian experience in grape accessions preservation, one of the most reliable ways is the creation of a duplicate in vitro collection. However, in connection with the creation of duplicate grape collections and development of genome editing techniques, there is a need for selecting the most optimal medium composition that will ensure the maximum rate of callus formation and regeneration during the introduction of grape plants into in vitro culture. This will make it possible to obtain the necessary amount of material for further editing and subsequent regeneration of plants with knockout of target genes to improve economically valuable traits. For grapes, this is primarily an increase in resistance to powdery mildew caused by Uncinula necator Burill.The effect of active substances of biological and synthetic origin on the morphogenesis has been studied for many crops, including regenerant plants of the genus Vitis L. However, the genus Vitis is very diverse and heterogeneous in its genetic, physiological and morphological structure, as a result of which the recommended media and components for cultivation under in vitro conditions may not suit every cultivar. Local Russian grape cultivars are better suited to local growing conditions, so efforts should be focused on the development of techniques related to the preservation of local varieties in collections in vitro.Knowledge of genes controlling certain traits, as well as the availability of grape accessions whose genome has been sequenced, contribute to successful in silico analysis for creating editing constructs.