Abstract
To investigate the in vitro anti-viral effect of fructopyrano-(1&rarr;4)-glucopyranose (FG) from traditional Chinese medicine Radix isatidis, dog kidney cells (MDCK) were with treated with either FG and then with virus (Group A); virus and then with FG (Group B); or FG and virus simultaneously (Group C). Results showed the in vitro cell protection rate and virus inhibition rate increased significantly with the increase of FG concentration when compared with cells without FG treatment (p<0.01). The anti-viral effect was the best in Group C and that in Group A was superior to that in Group B. FG could exert significant inhibited effect on neuraminidase activity with the IC50 of 0.07 mmol/mL and on hemagglutinin of avian influenza virus with minimum inhibitory concentration of 3.13 mg/mL. Our findings indicate FG can confer obvious antiviral effects on influenza A virus in vitro.
Highlights
Radix isatidis (Banlangen in Chinese), dried roots of Isatis indigotica Fort, is a valuable Chinese materia medica (CMM) with antibacterial and anti-viral activities, improving immune function, anti-tumor effect, etc
When the concentration of FG was lower than 12.50 mg/mL, there was no significant difference in cell proliferation between the FG group and control group, indicating that FG at
Sensitively and accurately evaluate the anti-viral effect of FG, MTT assay was performed and FG was used for prevention and treatment of virus infection
Summary
Radix isatidis (Banlangen in Chinese), dried roots of Isatis indigotica Fort, is a valuable Chinese materia medica (CMM) with antibacterial and anti-viral activities, improving immune function, anti-tumor effect, etc. It is applied in the treatment of infections, especially viral infections. With the wide application of natural medicine, the pharmacological activities of R. isatidis and its active ingredients have been studied indepth. Among the numerous active ingredients, fructopyrano-(1→4)-glucopyranose (FG) is a compound with potent immune activity and abundant in R. isatidis. Preliminary study showed the anti-viral activity of FG in vitro. The anti-viral activity of FG was evaluated by using a variety of measures
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