&lt;i&gt;In vitro &lt;/i&gt;anthelmintic effects of &lt;i&gt;Artemisia afra&lt;/i&gt; and &lt;i&gt;Mentha longifolia&lt;/i&gt; against parasitic gastro-intestinal nematodes of livestock

Nthatisi Innocentia Molefe,Ana Mbokeleng Tsotetsi,Oriel Matlhahane Molefi Thekisoe,Anofi Omotayo Tom Ashafa

doi:10.3329/bjp.v7i3.11437

In vitro anthelmintic effects of Artemisia afra and Mentha longifolia against parasitic gastro-intestinal nematodes of livestock

Nthatisi Innocentia Molefe, Ana Mbokeleng Tsotetsi + Show 2 more

Open Access

https://doi.org/10.3329/bjp.v7i3.11437

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Abstract

Gastrointestinal nematodes are the most widespread and economically important class of the helminths. The major threat posed by nematode infections is the productivity loss due to their ability to disrupt nutrient utilization of the livestock resulting in hunger and malnutrition. Acetone and aqueous extracts were prepared from Mentha longifolia and Artemisia afra and tested on egg hatch, larval development and larval mortality assays at three concentrations 2.5, 5.0 and 7.5 mg/mL. Certain percentages of egg hatch and larval mortality were inhibited by both plants; however, larval development was totally inhibited at all concentrations as compared to the negative control. Generally the acetone extracts from the two plants were most effective against parasitic gastrointestinal nematodes of livestock. This report therefore validates the folkloric use of these species in the management of internal parasites by the Basotho of the eastern Free State Province of South Africa.

Highlights

Gastrointestinal nematodes are mostly known to invade the digestive tract, the abomasums or even the small intestines (Maphosa and Masika, 2009)
The overall aim of the present study was to investigate the anthelmintic activity of A. afra and M. longifolia in order to validate their use in ethnoveterinary medicine of the eastern Free State Province of South Africa
At 2.5 mg/mL the water extract exhibited 13.5% while the acetone extract at the same concentration showed 30.1% inhibition of nematode egg hatchability (Table I)

Summary

Introduction

Gastrointestinal nematodes are mostly known to invade the digestive tract, the abomasums or even the small intestines (Maphosa and Masika, 2009). They are the worst parasites for livestock production worldwide (Diehl et al, 2004). They totally depend on their host for nourishment, nutrition and protection (Tsotetsi and Mbati, 2003). Small stock is the most susceptible to gastrointestinal nematode infections (Tsotetsi and Mbati, 2003)

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