Abstract
In the present experiment, the effects of a mutant strain Bacillus pumilus FF1-2 to defend the infection of Aeromonas hydrophilaon on Gibel carp were examined, which was got by mutation breeding from the original strain Bacillus pumilus F3-1 that could inhibit the quorum sensing molecule N-acyl-homoserine lactones (AHLs) in the previous study. The results of safety evaluation indicated that it was safe to the Gibel carp (Carassiusauratusgibelio) when the intraperitoneal injection concentration of mutation strain B. PumilusFF1-2 reached 1×1011CFU/ml in one-week experiment, and the sections of muscle, liver, kidney and heart didn’t show any histopathological characterization. The results of injection challenge showed that the B. Pumilus FF1-2 could protect the Gibel carp from the infection of A. hydrophila JY-1 when they were co-injected intraperitoneally with equal concentration of 0.5 ml 1×108 CFU/ml and the survival of Gibel carp was 68% (P<0.05); what’s more, the B. Pumilus FF1-2 could significantly increase the survival of Gibel carp than that of original strain of B. Pumilus F3-1 (P<0.05). In the immersion challenge experiment, the survival of Gibel carp in the B. Pumilus FF1-2 group was 70% when the Gibel carp bathed in the concentration of 1×108 CFU/ml of A. hydrophilaYJ-1 and B. Pumilus FF1-2, which was significantly higher than that of the B. Pumilus F3-1 as well (P<0.05). So, it was supposed that the mutant B. Pumilus FF1-2 could be used as the potential alternative to antibiotics to defend the infection of A. hydrophila in aquaculture.
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