Abstract
Effect of the leaves on the weight, urine output, and plasma and urine chemistry of salt-loaded rats was investigated. The control group received a diet consisting 100% of the commercial feed; the test-control received a diet consisting 8% salt and 92% commercial feed, while the test received diet containing 8% salt, 5% leaf powderand 87% commercial feed. There was no significant difference in the food intake of the three groups, although the weight change of the test animals was significantly lower (p
Highlights
We investigated the effect of the leaves on the weight, urine output, plasma and urine chemistry of salt-loaded rats
There was no significant difference in the food intake of the three groups, the weight change of the test animals was significantly lower (p
There was no significant difference in the daily mean daily urine output of the test and test –control, both of which were significantly higher (p
Summary
Procurement of Experimental Animals and Feed: Albino rats were collected from the animal house of. After due identification at the Department of Plant Science and Biotechnology, Faculty of Life sciences, University of Benin, Benin City, Nigeria, they were rid of dirt, oven dried and ground into powder and used for compounding the test diet. Experimental Design and Composition of Diet: The rats were randomly sorted into three groups of five animals each, so that the average weight difference was ±1.3g. The urine samples were collected and analyzed. At the end of the treatment period the animals were fasted overnight after which they were painlessly sacrificed by decapitation under chloroform anesthesia, and their blood collected into heparin sample bottles. The values of the various parameters for the control, test control and test groups were analyzed for statistical significant differences using the student’s t-test. Means which differ significantly at p=0.05, were assumed to be significantly different
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