<em>In vitro</em> glucuronidation of <em>trans</em>-Piceid and <em>trans</em>-Piceatannol by human liver microsomes

Abstract

<p style="text-align: justify;"><strong>Aims</strong> : The aim of the present investigation was to establish glucuronidation of trans-resveratrol derivates in the liver. Stilbenes are naturally occurring polyphenolic compounds which have been reported to have potential preventive activities in human diseases. Trans-stilbenes, mainly found in grapes and red wine, are important in terms of biological activities. However, little is known about the metabolism of these compounds in human.</p><p style="text-align: justify;"><strong>Methods and results</strong> : The glucuronoconjugation of stilbenes was investigated using human liver microsomes and the structure of new metabolites was characterized by LC-MS and proton NMR. For the first time, the structure of the metabolites of trans-piceid and trans-piceatannol was established. The reaction led to the formation of two glucuronides for trans-piceid and three for trans-piceatannol.</p><p style="text-align: justify;"><strong>Significance and impact of study</strong>: This study is of particular relevance since the phenolic substances of red wine (especially stilbenes) might be responsible for the potential beneficial effects of moderate and regular wine consumption.</p>