Abstract
The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 μM 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis.
Highlights
Sugarcane (Saccharum spp., Poaceae) is an important agricultural crop which is capable of producing valuable by-products such as sugar, biofuel, biofibers, wax and bioplastic (Singh, Kumar, Tiwari, Rastogi, & Singh, 2013)
When compared to other treatments, the longest stem occurred in plantlets cultivated under B:R=50:50 light emitting diodes (LEDs) light
The highest total fresh mass occurred in B:R=70:30 and 50:50 LED combinations, and in the FL
Summary
Sugarcane (Saccharum spp., Poaceae) is an important agricultural crop which is capable of producing valuable by-products such as sugar, biofuel, biofibers, wax and bioplastic (Singh, Kumar, Tiwari, Rastogi, & Singh, 2013). Micropropagation techniques have facilitated the rapid multiplication of newly developed varieties and assured the production of high quality and disease-free plantlets (Snyman, Meyer, Koch, Banasiak, & Watt, 2011). High production cost is a very important issue and the development of new technologies is required to such an end. Innovations, such as the use of light emitting diodes (LEDs) instead of white fluorescent lamps, have given satisfactory results for a variety of plants cultivated in vitro like cotton, anthurium, rapeseed and banana (Budiarto, 2010; Li, Xu, & Tang, 2010; Li, Tang, & Xu, 2013; Vieira et al, 2015)
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