Abstract
Studies on new microbial sources of cellulase and accurate assessment of the steps that increase cellulase production are essential strategies to reduce costs of various processes using such enzymes. This study aimed at the selection of cellulase-producing filamentous fungi, and at the research of parameters involving cellulase production by submerged fermentation. The first test consisted of selecting the best cellulase-producing microorganisms (FPase) in Erlenmeyer flasks containing 200 mL of specific growth medium. The next test was designed to further investigate the enzyme production in fermentation with four types of soluble sugars: glucose, lactose, sucrose and xylose. In bioreactor tests, three different inoculation strategies were analyzed. The best FPase activity was presented by the strain Trichoderma sp. CMIAT 041 (49.9 FPU L -1 ) and CMCase by the fungus Lasiodiplodia theobromae CMIAT 096 (350.0 U L -1 ). Sucrose proved to be the best option among the soluble sugars tested, with higher rates of FPase activity (49.9 FPU L -1 ) and CMCase (119.7 U L -1 ). The best inoculation strategy for the bioreactor was a spore suspension obtained from a semi-solid state fermentation of wheat bran for 72h.
Highlights
The intensive search for alternative energy sources has gained prominence since the 1970’s, when considerable research about bioethanol produced from biomass was developed (GUSAKOV, 2011)
Stock cultures were maintained on Potato Dextrose Agar (PDA) slants at 4°C
CMIAT 041 presented the highest activity of FPase after 72h of fermentation (49.9 FPU L-1 ± 0.013a) followed by Trichoderma sp
Summary
The intensive search for alternative energy sources has gained prominence since the 1970’s, when considerable research about bioethanol produced from biomass was developed (GUSAKOV, 2011). Biological Science growing need for alternative non-fossil energy, the synthesis of bioproducts and bioenergy generation, from renewable raw material such as lignocellulosic materials, at low cost, is important for sustainable development (DUTTA et al, 2008). In this scenario, stand out bioethanol and biodiesel produced from renewable raw material, and are known to reduce emissions of carbon monoxide in the atmosphere (ASHBY et al, 2005).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
