&lt;b&gt;Production of cellulases by &lt;i&gt;Penicillium oxalicum&lt;/i&gt; through solid state fermentation using agroindustrial substrates

Fabrícia Vieira Silva Bomtempo,Emerson Adriano Guarda,Deyla Paula De Oliveira,Franciane Maria Mantovani Santin,Raphael Sanzio Pimenta

doi:10.4025/actascibiolsci.v39i3.32384

<b>Production of cellulases by <i>Penicillium oxalicum</i> through solid state fermentation using agroindustrial substrates

Fabrícia Vieira Silva Bomtempo, Emerson Adriano Guarda + Show 3 more

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https://doi.org/10.4025/actascibiolsci.v39i3.32384

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Abstract

The purpose of this study was to define the factors that influence the production of cellulases by Penicillium oxalicum , a strain obtained from a leaf-cutting ant colony and identified based on the ITS gene . The experimental design included solid state fermentation using sugarcane bagasse and lignocellulosic sorghum as the lignocellulosic substrate. The variables were analyzed using a 2 5-1 fractional factorial design, with three replicates on the central point. All the variables analyzed influenced the production of at least one of the three cellulose types analyzed. The highest values observed were: FPase 4.2 U g -1 , CMCase 9.2 U g -1 and Avicelase 8.4 U g -1 using lignocellulosic sorghum as the substrate. The best conditions for enzyme production were: incubation temperature at 40oC, initial moisture of 60%, pH of 4.0 and a growth period of four days using lignocellulosic sorghum as the substrate.

Highlights

Lignocellulose is the most abundant organic compound in the biosphere; only a small amount produced in agriculture or forestry is used, the rest being considered waste, causing consequent deterioration of the environment (Sánchez, 2009)
This study molecularly identified the F33805 strain from the Coleção de Culturas Carlos Rosa, Universidade Federal do Tocantins
The sequence, which was generated using the ITS gene, shows 98% similarity with the strain Penicillium oxalicum (GenBank Accession LT558936.1) (Guevara-Suarez et al, 2016). It was identified as Penicillium oxalicum and its identity was confirmed in the CBS Database http://www.westerdijkinstitute.nl/ collections/), where it presented 99% similarity (IHEM Accession 2931)

Summary

Introduction

Lignocellulose is the most abundant organic compound in the biosphere; only a small amount produced in agriculture or forestry is used, the rest being considered waste, causing consequent deterioration of the environment (Sánchez, 2009). Much is being done to reduce losses of this resource and to diminish the resulting environmental degradation (Ballesteros, 2001) through the generation of a series of high-value products and byproducts such as cellulolytic enzymes and cellulosic ethanol (Isikgor & Becer, 2015). Some microorganisms, like Penicillium oxalicum are able to degrade this lignocellulosic material using hydrolytic and oxidative enzymes naturally produced by the microorganisms themselves (Xian, Wang, Yin, & Feng, 2016). These enzymes include cellulases, the main recruitable resource for the bioconversion of cellulosics to useful products, and usually the most costly part of the production process. For example, the cost of cellulases is about 40% of the total cost of production

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